[12843 Search Results


pta 12843  (ATCC)
94
ATCC pta 12843
Pta 12843, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qiagen cat  (Addgene inc)
93
Addgene inc qiagen cat
Qiagen Cat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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12843s  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc 12843s
12843s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against nek3  (Proteintech)
91
Proteintech antibodies against nek3
Quantitative RT-PCR to detect NEKs gene expression in esophageal cell lines. qRT-PCR was performed as described in the Methods section. The gene expression of NEKs is presented as relative gene expression normalized to the HPRT1 of the same samples. NEK1 ( A ), NEK2 ( B ), <t>NEK3</t> ( C) , NEK4 ( D ), NEK5 ( E ), NEK6 ( F ), NEK7 ( G ), NEK8 ( H ), NEK9 ( I ), NEK10 ( J ) and NEK11 ( K ) are shown. HEEC, normal esophagus; BART and CPA, Barrett’s esophagus; CPB, dysplastic Barrett’s; FLO1, OE19, OE33, SKGT4, and OAC M5.1, esophageal adenocarcinoma. Results were compared to the HEEC, a normal control. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.
Antibodies Against Nek3, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fga h-12843  (Shanghai Hengyuan Trading)
90
Shanghai Hengyuan Trading fga h-12843
Validated expression of potential serum biomarkers by ELISA and western blot. ( A – G ) ELISA analysis <t>of</t> <t>ACLY,</t> <t>FGA</t> and APOA1 in the serum of different groups. ( A – C ) ACLY, FGA and APOA1 expression in perioperative group. (d-e) ACLY and FGA expression in AR group. ( F – G ) ACLY and FGA expression in ITBL group. (*** p < 0.001, ** p < 0.01, * p < 0.05) ( H – J ) Confirmation of proteomic results by western blot in the serum of different groups. ( H ) Detection of ACLY, FGA and APOA1 in perioperative group, randomly chose three serum samples from pre-transplants, post-transplants and healthy controls, respectively. ( I ) Detection of ACLY and FGA in AR group, randomly chose three serum samples from ARs, transplant excellents and healthy controls, respectively. ( J ) Detection of ACLY and FGA in ITBL group, randomly chose three serum samples from ITBLs, transplant excellents and healthy controls, respectively. Transferred proteins to the PVDF membrane were detected by Ponceau S stain as western blot loading control.
Fga H 12843, supplied by Shanghai Hengyuan Trading, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fga h-12843 - by Bioz Stars, 2026-02
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Image Search Results


Quantitative RT-PCR to detect NEKs gene expression in esophageal cell lines. qRT-PCR was performed as described in the Methods section. The gene expression of NEKs is presented as relative gene expression normalized to the HPRT1 of the same samples. NEK1 ( A ), NEK2 ( B ), NEK3 ( C) , NEK4 ( D ), NEK5 ( E ), NEK6 ( F ), NEK7 ( G ), NEK8 ( H ), NEK9 ( I ), NEK10 ( J ) and NEK11 ( K ) are shown. HEEC, normal esophagus; BART and CPA, Barrett’s esophagus; CPB, dysplastic Barrett’s; FLO1, OE19, OE33, SKGT4, and OAC M5.1, esophageal adenocarcinoma. Results were compared to the HEEC, a normal control. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

Journal: Cancers

Article Title: Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett’s Esophagus

doi: 10.3390/cancers15194821

Figure Lengend Snippet: Quantitative RT-PCR to detect NEKs gene expression in esophageal cell lines. qRT-PCR was performed as described in the Methods section. The gene expression of NEKs is presented as relative gene expression normalized to the HPRT1 of the same samples. NEK1 ( A ), NEK2 ( B ), NEK3 ( C) , NEK4 ( D ), NEK5 ( E ), NEK6 ( F ), NEK7 ( G ), NEK8 ( H ), NEK9 ( I ), NEK10 ( J ) and NEK11 ( K ) are shown. HEEC, normal esophagus; BART and CPA, Barrett’s esophagus; CPB, dysplastic Barrett’s; FLO1, OE19, OE33, SKGT4, and OAC M5.1, esophageal adenocarcinoma. Results were compared to the HEEC, a normal control. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

Article Snippet: The same tissue microarray from TissueArray.com ES8011b was used for immunofluorescence staining utilizing antibodies against NEK3 (12843-1-AP, Proteintech) and NEK7 (B5, Santa Cruz).

Techniques: Quantitative RT-PCR, Gene Expression, Control

Quantitative RT-PCR to detect NEKs gene expression in primary tissue samples from normal esophagus (NE) and esophageal adenocarcinoma (EAC). qRT-PCR was performed as described in the Methods section. NEK1 ( A ), NEK2 ( B ), NEK3 ( C ), NEK4 ( D ), NEK6 ( E ), NEK7 ( F ), NEK8 ( G ), NEK9 ( H ) and NEK11 (I) are presented. The gene expression of NEKs are presented as relative gene expression fold normalized to the HPRT1 of the same samples. *, p < 0.05; **, p < 0.01; ns, not significant.

Journal: Cancers

Article Title: Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett’s Esophagus

doi: 10.3390/cancers15194821

Figure Lengend Snippet: Quantitative RT-PCR to detect NEKs gene expression in primary tissue samples from normal esophagus (NE) and esophageal adenocarcinoma (EAC). qRT-PCR was performed as described in the Methods section. NEK1 ( A ), NEK2 ( B ), NEK3 ( C ), NEK4 ( D ), NEK6 ( E ), NEK7 ( F ), NEK8 ( G ), NEK9 ( H ) and NEK11 (I) are presented. The gene expression of NEKs are presented as relative gene expression fold normalized to the HPRT1 of the same samples. *, p < 0.05; **, p < 0.01; ns, not significant.

Article Snippet: The same tissue microarray from TissueArray.com ES8011b was used for immunofluorescence staining utilizing antibodies against NEK3 (12843-1-AP, Proteintech) and NEK7 (B5, Santa Cruz).

Techniques: Quantitative RT-PCR, Gene Expression

Immunohistochemistry (IHC) and immunofluorescence (IF) staining of NEKs in a tissue microarray (TMA) containing normal esophagus (NE) and EAC . ( A , B ) IHC of NEK1 in a representative NE ( A ) and EAC ( B ). ( C ) A summary of the IHC results of NEK1 from the TMA. ( D , E ) IHC of NEK2 in a representative NE ( D ) and EAC ( E ). ( F ) A summary of the IHC results of NEK2 from the TMA. (G-H) IF staining of NEK3 in a representative NE ( G ) and EAC ( H ). ( I ) A summary of the IF results of NEK3 from the TMA. (J–K) IF staining of NEK7 in a representative NE ( J ) and EAC ( K ). ( L ) A summary of the IF results of NEK7 from the TMA. **, p < 0.01; ****, p < 0.0001.

Journal: Cancers

Article Title: Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett’s Esophagus

doi: 10.3390/cancers15194821

Figure Lengend Snippet: Immunohistochemistry (IHC) and immunofluorescence (IF) staining of NEKs in a tissue microarray (TMA) containing normal esophagus (NE) and EAC . ( A , B ) IHC of NEK1 in a representative NE ( A ) and EAC ( B ). ( C ) A summary of the IHC results of NEK1 from the TMA. ( D , E ) IHC of NEK2 in a representative NE ( D ) and EAC ( E ). ( F ) A summary of the IHC results of NEK2 from the TMA. (G-H) IF staining of NEK3 in a representative NE ( G ) and EAC ( H ). ( I ) A summary of the IF results of NEK3 from the TMA. (J–K) IF staining of NEK7 in a representative NE ( J ) and EAC ( K ). ( L ) A summary of the IF results of NEK7 from the TMA. **, p < 0.01; ****, p < 0.0001.

Article Snippet: The same tissue microarray from TissueArray.com ES8011b was used for immunofluorescence staining utilizing antibodies against NEK3 (12843-1-AP, Proteintech) and NEK7 (B5, Santa Cruz).

Techniques: Immunohistochemistry, Immunofluorescence, Staining, Microarray

Analyses of associations of NEKs gene expression and patients’ survival . An online tool, Kaplan–Meier Plotter ( http://kmplot.com , accessed on 12 June 2023), was used to analyze the association between NEKs gene expression levels and EAC patients’ overall survival, according to the instructions of the website. NEK1 ( A ), NEK2 ( B ), NEK3 ( C ), NEK4 ( D ), NEK5 ( E ), NEK6 ( F ), NEK7 ( G ), NEK8 ( H ), NEK9 ( I ), NEK10 ( J ) and NEK11 ( K ) are shown.

Journal: Cancers

Article Title: Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett’s Esophagus

doi: 10.3390/cancers15194821

Figure Lengend Snippet: Analyses of associations of NEKs gene expression and patients’ survival . An online tool, Kaplan–Meier Plotter ( http://kmplot.com , accessed on 12 June 2023), was used to analyze the association between NEKs gene expression levels and EAC patients’ overall survival, according to the instructions of the website. NEK1 ( A ), NEK2 ( B ), NEK3 ( C ), NEK4 ( D ), NEK5 ( E ), NEK6 ( F ), NEK7 ( G ), NEK8 ( H ), NEK9 ( I ), NEK10 ( J ) and NEK11 ( K ) are shown.

Article Snippet: The same tissue microarray from TissueArray.com ES8011b was used for immunofluorescence staining utilizing antibodies against NEK3 (12843-1-AP, Proteintech) and NEK7 (B5, Santa Cruz).

Techniques: Gene Expression

Validated expression of potential serum biomarkers by ELISA and western blot. ( A – G ) ELISA analysis of ACLY, FGA and APOA1 in the serum of different groups. ( A – C ) ACLY, FGA and APOA1 expression in perioperative group. (d-e) ACLY and FGA expression in AR group. ( F – G ) ACLY and FGA expression in ITBL group. (*** p < 0.001, ** p < 0.01, * p < 0.05) ( H – J ) Confirmation of proteomic results by western blot in the serum of different groups. ( H ) Detection of ACLY, FGA and APOA1 in perioperative group, randomly chose three serum samples from pre-transplants, post-transplants and healthy controls, respectively. ( I ) Detection of ACLY and FGA in AR group, randomly chose three serum samples from ARs, transplant excellents and healthy controls, respectively. ( J ) Detection of ACLY and FGA in ITBL group, randomly chose three serum samples from ITBLs, transplant excellents and healthy controls, respectively. Transferred proteins to the PVDF membrane were detected by Ponceau S stain as western blot loading control.

Journal: Aging (Albany NY)

Article Title: Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients

doi: 10.18632/aging.103381

Figure Lengend Snippet: Validated expression of potential serum biomarkers by ELISA and western blot. ( A – G ) ELISA analysis of ACLY, FGA and APOA1 in the serum of different groups. ( A – C ) ACLY, FGA and APOA1 expression in perioperative group. (d-e) ACLY and FGA expression in AR group. ( F – G ) ACLY and FGA expression in ITBL group. (*** p < 0.001, ** p < 0.01, * p < 0.05) ( H – J ) Confirmation of proteomic results by western blot in the serum of different groups. ( H ) Detection of ACLY, FGA and APOA1 in perioperative group, randomly chose three serum samples from pre-transplants, post-transplants and healthy controls, respectively. ( I ) Detection of ACLY and FGA in AR group, randomly chose three serum samples from ARs, transplant excellents and healthy controls, respectively. ( J ) Detection of ACLY and FGA in ITBL group, randomly chose three serum samples from ITBLs, transplant excellents and healthy controls, respectively. Transferred proteins to the PVDF membrane were detected by Ponceau S stain as western blot loading control.

Article Snippet: All serum samples were analyzed blindly in triplicate and the concentrations of ACLY (Shanghai Hengyuan, H-12925), FGA (Shanghai Hengyuan Biotech, H-12843) and APOA1 (Shanghai Hengyuan, H-11508) were measured according to the manufacturer’s instructions respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Staining

Correlation analysis between serum potential biomarkers and clinical information in perioperative group.

Journal: Aging (Albany NY)

Article Title: Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients

doi: 10.18632/aging.103381

Figure Lengend Snippet: Correlation analysis between serum potential biomarkers and clinical information in perioperative group.

Article Snippet: All serum samples were analyzed blindly in triplicate and the concentrations of ACLY (Shanghai Hengyuan, H-12925), FGA (Shanghai Hengyuan Biotech, H-12843) and APOA1 (Shanghai Hengyuan, H-11508) were measured according to the manufacturer’s instructions respectively.

Techniques: